ʻO nā mea e hoʻoholo ai i ka pono o ka synthesis DNA

I ka DNA maʻamau, RNA a me nā ʻakika nucleic non-natural 'synthesis, ʻo ka Deprotection and Coupling step ka hana koʻikoʻi.

ʻO ka ʻanuʻu Deprotection ka wehe ʻana i ka pūʻulu DMT ma ke kākoʻo paʻa a i ʻole ka hui hydroxyl 5' ma ka nucleoside mua me ka waikawa organik, a hōʻike i ka pūʻulu hydroxyl no ka pae hoʻohui e hiki mai ana.ʻO ka 3% trichloroacetic acid i loko o ka dichloromethane a i ʻole toluene e hoʻohana nui ʻia e hana i ka hana deprotection.ʻO ka manaʻo o ka trichloroacetic acid a me ka manawa deprotection (deblocking time) e hoʻomalu i ka maʻemaʻe o nā huahana hope.ʻO ka haʻahaʻa haʻahaʻa a me ka lawa ʻole o ka manawa deblocking e waiho i ka hui DMT unreacted, e hōʻemi ana i ka hua a hoʻonui i nā mea haumia ʻole.Hiki i ka manawa deblocking lōʻihi ke alakaʻi i ka depurine o nā kaʻina synthesized, e hana ana i nā haumia i manaʻo ʻole ʻia.

Hoʻopili ka ʻanuʻu Coupling i ka ʻike wai o nā mea hoʻoheheʻe a me ka wai i ka lewa.Pono e emi ka nui o ka wai i ka synthesis ma mua o 40 ppm, ʻoi aku ka maikaʻi ma mua o 25 ppm.No ka mālama ʻana i ke kūlana synthesis anhydrous, pono e hoʻokō ʻia ka synthesis nucleic acids i kahi haʻahaʻa haʻahaʻa haʻahaʻa, no laila ke paipai nei mākou i kā mākou mea kūʻai aku e hoʻohana i kaNā lako i hoʻoheheʻe ʻia ʻo Amidites, hiki ke hoʻoheheʻe i ka Phosphoramidite pauka a ʻaila i loko o ka acetonitrile anhydrous e pale aku i ka pili ʻana me ka ea.