Nā pahele molekala no ka phosphoramidite a me nā reagents Kiʻi Hōʻikeʻike

Nā pahele molekula no ka phosphoramidite a me nā reagents

Noi:

Hoʻohana ʻia ka Molecular Trap e hoʻopili i ka wai i loko o nā reagents a me ka amidite, ua hana mua ʻia no ka synthesis o oligonucleotides.He maʻalahi, ʻaʻohe lepo, a ʻaʻohe flannel.Hiki ke hoʻohui ʻia i nā ʻano mea hoʻoheheʻe a me nā mea hoʻonā organik e wehe i ka nui o ka wai.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Hōʻike huahana

I ka ABI a me MilliGen/PerSeptive DNA synthesis hoʻāʻo, hiki i ka sub-sieve pack ke mālama i ka ʻike wai i loko o ka nitrile a me nā ʻōmole activator ma lalo o 10 ppm, me ka hopohopo ʻole e pili ana i ka valve a i ʻole ka puʻu throttle i māhele ʻia i ka sub-sieve.Hoʻopaʻa ʻia ka lepo a i ʻole ka lint ma ka ʻeke ʻeke.Hiki ke hoʻohana pololei ʻia no ka hoʻoheheʻe ʻana o 500 ml, 1L, 2 L a me nā ʻōmole solvent ʻē aʻe, a hiki ke hana ʻia nā kikoʻī sub-sieve pack.

Hōʻike kēia i ka hopena hoʻoheheʻe ikaika o 10 g molecular trap ma 500ml, 1L, 4L nitrile samples me nā ʻano wai like ʻole.

E hoʻohālikelike me ka pahele molecular import

500 ml 197 ppm ACN
Manawa(h)	0	24	48	72	96
HonyaBio	197	33	16.5	6.5	6
Nā ʻāina ʻē aʻe	197	43	27	15	15

1 L 143 ppm ACN
Manawa(h)	0	24	48	72	96
HonyaBio	143	48	32	20	15
Nā ʻāina ʻē aʻe	142	47	36	23	15

4 L 141 ppm ACN
Manawa(h)	0	24	48	72	96
HonyaBio	141	95	94	84	73
Nā ʻāina ʻē aʻe	141	96	95	85	72

Nā kuhikuhi a me ka hoʻohana

Hoʻopili ʻia ka pahele molekala, a pono e wehe ʻia inā hoʻohana ʻia, a pono e hōʻoia ʻia ka sila o ka hue reagent i ka wā e hoʻohana ai.
2g kānana no 24 h hiki ke hoʻemi i ka 165 ppm wai i loko o 500 ml nitrile i 105 ppm.
5 g kānana no 24 h hiki ke hoʻemi i ka 172 ppm wai i loko o 500 ml nitrile i 58 ppm.
10 g kānana 24 h Hiki iā ia ke hoʻemi i ka 166 ppm wai i loko o 1 L nitrile i 68 ppm.
Hiki i ka 20 g sub-sieve ke hoʻemi i ka 162 ppm wai i loko o 4 L nitrile i 109 ppm no 24 mau hola.

Manaʻo mākou i 2 g subsieve no nā ʻōmole reagent 50-250ml, 5g no nā hue reagent 250-500ml, 10g no nā ʻōmole reagent 500-1000ml, a me 20g no nā hue reagent 1000-2000ml.

Hōʻoiaʻiʻo

Loaʻa i kēlā me kēia pūʻolo sub-screen ka mana koʻikoʻi i ka wā o ke kaʻina hana.A he mea pono i nā mea kūʻai ke nānā i ka pahu ma mua o ka hoʻohana ʻana:

ʻO ka mea mua, e hōʻoia i ka paʻa ʻole o ka pahu pahu.ʻO kēlā me kēia leaka leaka a i ʻole ke komo ʻana o ka ea e hōʻemi i ka hana o ka huahana.

ʻO ka lua, e makaʻala i ka wā e wehe ai i ka pahu e pale ai i ke kiʻi ʻoniʻoni sub-screen mai ʻoki ʻia, a e nānā inā he leak ke kiʻiʻoniʻoni.

Lapaʻau ma hope o ka hoʻohana ʻana

ʻAʻole ia he mea ʻawaʻawa a pōʻino paha, e hoʻohaumia ʻia lākou e nā kemika a me nā mea hoʻoheheʻe a lākou e launa pū ai a pono e hoʻolei ʻia ma ke ʻano he ʻōpala haumia ma hope o ka hoʻohana ʻana.

Hoʻohālike a me ka noi

Ua hoʻolako mua mākou i nā ʻeke sub-sieve i ka nui o 2 g, 5 g, 10 g, a me 20 g., A hiki ke hoʻopili ʻia nā kikoʻī ʻē aʻe e like me nā pono o ka mea kūʻai aku.

He kūpono ka pahele mole no ka hoʻolaha lōʻihi i ka nitrile, a hiki ke hoʻohana ʻia no ka acetic acid, ether, acetic ether, butyl acetate, alcohol, isopropanol, methanol, butanol, Phenol, pyridine, hydrochloric acid, nitric acid, phosphoric acid. , sulfuric acid, methyl chloride, nitrogen methyl imidazole, etc.

ʻAʻole kūpono paha ia no ka ʻike lōʻihi i ka tetrahydrofuran, toluene, methyl formamide (DMF), methyl methyl amide (DMAc), N-methylpyrrolidone (NMP) a me nā mea hoʻonā ʻē aʻe.